اثر تزریق داخل بطنی سالوبرینال، یک مهار کننده جدید سنتز پروتئین، بر تشنجات ناشی از پنتیلن تترازول در موش صحرایی نر

Background and Objective: Salubrinal- a protein synthesis inhibitor- plays a crucial role in sleep and neuronal inhibition through the GABA activation. According to these evidences, the aim of the present study was to determine the effect of salubrinal on epileptic seizures induced by pentylenetetrazol (PTZ). Materials and Methods: In this expremental study under stereotaxical surgery, three monopolar electrodes were fixed on the skull bone of animals and a cannula was implanted in the right cerebral ventricle according to Paxinos coordinates. After 7 days of recovery, each animal received intracerebroventricular (ICV) vehicle, diazepam (0.2 mg/rat) or salubrinal (25 or 50 µg/rat) 10 minutes before Pentylenetetrazol injection (subcutaneous, 60mg/kg). Electrophysiological and behavioral parameters of induced seizures were recorded during 60 minutes. Results : Salubrinal (25 and 50 µg/rat) significantly retarded the onset of generalized tonic-clonic seizures when compared to vehicle treatment (p<0.05). Epileptic discharge duration and ictal discharge duration were significantly shortened in salubrinal treated rats (p<0.05). Additionally, the rats treated with salubrinal (25 and 50 µg/rat) had lower seizure stage scores, as compared to vehicle treated rats (p<0.05). Conclusion : The findings of this study revealed that shortly after ICV. administration of salubrinal, epileptic seizures can be significantly decreased. Also, the findings suggest that protein synthesis is an essential process during development of epileptic seizures. Key words : Seizure, Salubrinal, Protein synthesis inhibitor, Rat, Pentylenetetrazol   Funding: This research was funded by Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Conflict of interest: None declared. Ethical approval: The Research Committee of Shahid Sadoughi University of Medical Sciences approved the study.   How to cite this article: Izadi M, Rezvani ME, Esmailidehaj M. Effects of Intracerebroventricular Administration of Salubrinal-A Protein Synthesis Inhibitor-on Epileptic Seizures Induced by Pentylenetetrazol in Male Rats. J Rafsanjan Univ Med Sci 2014 13(3): 281-92. [Farsi]